Biosearch Technologies Locked Nucleic Acid (LNA) Probes

Improve assay sensitivity and specificity with locked nucleic acid (LNA) oligonucleotides. LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, connecting the 2' oxygen atom to the 4' carbon atom, fixing it in the C3'-endo conformation.

LNAs enable a shorter probe, which supports assay design for:

• Improved assay sensitivity
  • Higher quenching efficiency (lower background)
  • Greater signal-to-noise ratio
• Increased hybridization specificity
• Targeting A-T rich sequences
• Better mismatch discrimination in SNP genotyping assays